Order of Draw Quiz

How the Scales are Structured
Who Usually Takes This Test?
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Below is a preview of how scores are typically distributed across each scale.
Example Questions From the Test
- To make every specimen clot at the same rate
- To ensure the largest tube is always filled first
- To reduce additive carryover that could alter later specimens
- To eliminate the need to mix additive tubes
- Green, gold, lavender
- Lavender, green, gold
- Gold, lavender, green
- Gold, green, lavender
- Gray, then lavender
- Either one; their additives cannot affect each other
- Lavender, then gray
- Collect another green tube before either one
Order of Draw Quiz - Description and methodology of the test
Designed for students, new phlebotomists, and clinicians refreshing collection technique, the order of draw quiz checks whether you can connect tube sequence with specimen quality. Its questions turn familiar collection rules into practical decisions, while the result highlights which areas deserve review before supervised practice or routine venipuncture. The questions apply phlebotomy collection rules to practical decisions, keeping the focus on applied knowledge rather than memorized labels.
What This Assessment Explores
The questionnaire uses multiple-choice scenarios rather than isolated definitions. Items ask you to choose a sequence, identify additive contamination, place a discard tube, or resolve priorities involving cultures, citrate, serum, heparin, EDTA, fluoride/oxalate, and special tubes.
Together, the questions cover these aspects of overall order-of-draw knowledge:
- Order of Draw Knowledge (0-16) gives one point for each correct answer and places performance in Foundation to review (0-6), Developing knowledge (7-12), or Strong knowledge (13-16).
- Correct sequencing shows whether major specimen types are positioned properly.
- Additive carryover connects a misplaced tube with effects such as falsely increased potassium or decreased calcium.
- Collection decisions cover cultures, winged sets, discard tubes, mixing, and trace-element additives.
For learners and practitioners, this blood tube sequence test checks procedural knowledge before supervised or routine collection; it does not measure dexterity or patient interaction.
Methodology and Scientific Basis
Each item presents one best answer, so the questionnaire uses a knowledge-check format rather than a symptom or personality scale. The options test recognition of tube placement and common collection exceptions.
Scoring is additive: each correct answer receives one point.
Its content is conceptually consistent with Calam and Cooper (1982), whose work links tube order with prevention of additive carryover. It also follows principles in CLSI GP41 and the EFLM Working Group opinion by Cornes and colleagues (2017), including priorities for cultures, citrate, serum, plasma, and anticoagulant tubes. Bazzano and colleagues (2021) provide later systematic context for recommendations and exceptions. The questionnaire is not an adaptation of any one source.
Displayed bands are educational interpretations and do not certify hands-on competence. Performance can vary with recall, prior training, guessing, and familiarity with local collection procedures. The scenarios emphasize recognition of standard sequences and common collection decisions. A venous collection knowledge test is most useful alongside current local procedures and supervised training.
- Roger A. Calam and Marsha H. Cooper. “Recommended ‘order of draw’ for collecting blood specimens into additive-containing tubes.” Clinical Chemistry, 28(6), 1399, 1982. 10.1093/clinchem/28.6.1399
- Clinical and Laboratory Standards Institute. Collection of Diagnostic Venous Blood Specimens. 7th ed. CLSI standard GP41. Wayne, PA: Clinical and Laboratory Standards Institute, 2017.
- Michael Cornes, Edmée van Dongen-Lases, Kjell Grankvist, Mercedes Ibarz, Gunn Kristensen, Giuseppe Lippi, Mads Nybo, and Ana-Maria Simundic. “Order of blood draw: Opinion Paper by the European Federation for Clinical Chemistry and Laboratory Medicine (EFLM) Working Group for the Preanalytical Phase (WG-PRE).” Clinical Chemistry and Laboratory Medicine, 55(1), 27-31, 2017. 10.1515/cclm-2016-0426
- Giacomo Bazzano, Alessandro Galazzi, Gian Domenico Giusti, Mauro Panigada, and Dario Laquintana. “The Order of Draw during Blood Collection: A Systematic Literature Review.” International Journal of Environmental Research and Public Health, 18(4), 1568, 2021. 10.3390/ijerph18041568
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